Journal: Bioengineered

Article Title: Endothelial progenitor cells-derived exosomes transfer microRNA-30e-5p to regulate Erastin-induced ferroptosis in human umbilical vein endothelial cells via the specificity protein 1/adenosine monophosphate-activated protein kinase axis

doi: 10.1080/21655979.2022.2025519

Figure Lengend Snippet: EPCs-Exos could be transferred to HUVECs. EPCs-Exos were extracted and identified. The uptake of EPCs-Exos by HUVECs was observed. (a) Morphological changes of EPCs were observed using an inverted microscope; (b) EPCs markers CD31 and CD34 were detected by immunofluorescence; (c) FITC-UEA-I and Dil-ac-LDL were detected by immunofluorescence; (d) The ultrastructure of Exos were observed using a TEM; (e) The diameter distribution and concentration of Exos were determined by NTA; (f) Exos mark proteins CD9, CD63, CD81, and Calnaxin were detected by WB; (g) The entry of PKH67-labeled Exos into HUVECs was detected by immunofluorescence. The cell experiments were repeated 3 times independently.

Article Snippet: Primary antibodies were CD9 (1:1000, ab92726, Abcam), CD63 (1:1000, ab134045, Abcam), CD81 (1:1000, ab79559, Abcam), Calnaxin (1:1000, ab92356, Abcam), SP1 (1:1000, ab124804, Abcam), p-adenosine monophosphate-activated protein kinase (AMPK) (1:1000, ab133448, Abcam), AMPK (1:1000, ab32047, Abcam) and GAPDH (1:1000, ab9485, Abcam).

Techniques: Inverted Microscopy, Immunofluorescence, Concentration Assay, Labeling

Journal: Cell Reports

Article Title: Limited extent and consequences of pancreatic SARS-CoV-2 infection

doi: 10.1016/j.celrep.2022.110508

Figure Lengend Snippet:

Article Snippet: Anti-human CD9 (mouse IgG1, clone SN4 C3-3A2) - 171Yb , Fluidigm , Cat# 3171009B; RRID: AB_2877094.

Techniques: Conjugation Assay, Purification, Blocking Assay, Recombinant, Control, Virus, Saline, Modification, Staining, Library Quantification, Antibody Labeling, Flow Cytometry, Software, Cytometry, Sequencing